Rabbit model with vocal fold hyperadduction

Published:January 27, 2022DOI:



      Adductor spasmodic dysphonia (AdSD) is caused by hyperadduction of the vocal folds during phonation, resulting in a strained voice. Animal models are not yet used to elucidate this intractable disease because AdSD has a difficult pathology without a definitive origin. For the first step, we established an animal model with vocal fold hyperadduction and evaluated its validity by assessing laryngeal function.


      In this experimental animal study, three adult Japanese 20-week-old rabbits were used. The models were created using a combination of cricothyroid approximation, forced airflow, and electrical stimulation of the recurrent laryngeal nerves (RLNs). Cricothyroid approximation was added to produce a glottal slit. Thereafter, both RLNs were electrically stimulated to induce vocal fold hyperadduction. Finally, the left RLN was transected to relieve hyperadduction. The sound, endoscopic images, and subglottal pressure were recorded, and acoustic analysis was performed.


      Subglottal pressure increased significantly, and the strained sound was produced after the electrical stimulation of the RLNs. After transecting the left RLN, the subglottal pressure decreased significantly, and the strained sound decreased. Acoustic analysis revealed an elevation of the standard deviation of F0 (SDF0) and degree of voice breaks (DVB) through stimulation of the RLNs, and degradation of SDF0 and DVB through RLN transection. Formant bands in the sound spectrogram were interrupted by the stimulation and appeared again after the RLN section.


      This study developed a rabbit model with vocal fold hyperadduction . The subglottal pressure and acoustic analysis of this model resembled the characteristics of patients with AdSD. This model could be helpful to elucidate the pathology of the larynx caused by hyperadduction, and evaluate and compare the treatments for strained phonation.


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        • Ludlow CL.
        Spasmodic dysphonia: a laryngeal control disorder specific to speech.
        J Neurosci. 2011; 31: 793-797
        • Umeno H
        • Hyodo M
        • Haji T
        • Hara H
        • Imaizumi M
        • Ishige M
        • et al.
        A summary of the Clinical Practice Guideline for the Diagnosis and Management of Voice Disorders, 2018 in Japan.
        Auris Nasus Larynx. 2020; 47: 7-17
        • Tanner K
        • Roy N
        • Merrill RM
        • Sauder C
        • Houtz DR
        • Smith ME.
        Spasmodic dysphonia: onset, course, socioemotional effects, and treatment response.
        Ann Otol Rhinol Laryngol. 2011; 120: 465-473
        • Hyodo M
        • Hisa Y
        • Nishizawa N
        • Omori K
        • Shiromoto O
        • Yumoto E
        • et al.
        The prevalence and clinical features of spasmodic dysphonia: a review of epidemiological surveys conducted in Japan.
        Auris Nasus Larynx. 2021; 48: 179-184
        • Genack SH
        • Woo P
        • Colton RH
        • Goyette D.
        Partial thyroarytenoid myectomy: an animal study investigating a proposed new treatment for adductor spasmodic dysphonia.
        Otolaryngol Head Neck Surg. 1993; 108: 256-264
        • Berke GS
        • Blackwell KE
        • Gerratt BR
        • Verneil A
        • Jackson KS
        • Sercarz JA.
        Selective laryngeal adductor denervation-reinnervation: a new surgical treatment for adductor spasmodic dysphonia.
        Ann Otol Rhinol Laryngol. 1999; 108: 227-231
        • Gibbs SR
        • Blitzer A.
        Botulinum toxin for the treatment of spasmodic dysphonia.
        Otolaryngol Clin North Am. 2000; 33: 879-894
        • Isshiki N
        • Tsuji DH
        • Yamamoto Y
        • Iizuka Y.
        Midline lateralization thyroplasty for adductor spasmodic dysphonia.
        Ann Otol Rhinol Laryngol. 2000; 109: 187-193
        • Allegretto M
        • Morrison M
        • Rammage L
        • Lau DP.
        Selective denervation: reinnervation for the control of adductor spasmodic dysphonia.
        J Otolaryngol. 2003; 32: 185-189
        • Dedo HH.
        Recurrent laryngeal nerve section for spastic dysphonia.
        Ann Otol Rhinol Laryngol. 1976; 85: 451-459
        • Novaleski CK
        • Kojima T
        • Chang S
        • Luo H
        • Valenzuela CV
        • Rousseau B.
        Nonstimulated rabbit phonation model: cricothyroid approximation.
        Laryngoscope. 2016; 126: 1589-1594
        • Sanuki T
        • Yumoto E
        • Kodama N
        • Minoda R
        • Kumai Y.
        Long-term voice handicap index after type II thyroplasty using titanium bridges for adductor spasmodic dysphonia.
        Auris Nasus Larynx. 2014; 41: 285-289
        • Boutsen F
        • Cannito MP
        • Taylor M
        • Bender B.
        Botox treatment in adductor spasmodic dysphonia: a meta-analysis.
        J Speech Lang Hear Res. 2002; 45: 469-481
        • Mendelsohn AH
        • Berke GS.
        Surgery or botulinum toxin for adductor spasmodic dysphonia: a comparative study.
        Ann Otol Rhinol Laryngol. 2012; 121: 231-238
        • Woo P.
        Carbon dioxide laser-assisted thyroarytenoid myomectomy.
        Lasers Surg Med. 1990; 10: 438-443
        • Jiang JJ
        • Titze IR
        A methodological study of hemilaryngeal phonation.
        Laryngoscope. 1993; 103: 872-882
        • Kodama H
        • Kumai Y
        • Nishimoto K
        • Toya Y
        • Miyamaru S
        • Furushima S
        • et al.
        The ferret as a surgical model for vocal fold scar creation and treatment.
        Ann Otol Rhinol Laryngol. 2018; 127: 146-154
        • Ge PJ
        • French LC
        • Ohno T
        • Zealear DL
        • Rousseau B.
        Model of evoked rabbit phonation.
        Ann Otol Rhinol Laryngol. 2009; 118: 51-55
        • Nakagishi Y
        • Morimoto Y
        • Fujita M
        • Ozeki Y
        • Maehara T
        • Kikuchi M.
        Rabbit model of airway stenosis induced by scraping of the tracheal mucosa.
        Laryngoscope. 2005; 115: 1087-1092
        • Iravani K
        • Mehravar S
        • Bahador M
        • Azarpira N.
        The healing effect of amniotic membrane in laryngeal defects in rabbit model.
        Laryngoscope. 2021; 131 (E527–33-e33)
        • Swanson ER
        • Abdollahian D
        • Ohno T
        • Ge P
        • Zealear DL
        • Rousseau B.
        Characterization of raised phonation in an evoked rabbit phonation model.
        Laryngoscope. 2009; 119: 1439-1443
        • Rojas GVE
        • Ricz H
        • Tumas V
        • Rodrigues GR
        • Toscano P
        • Aguiar-Ricz L.
        Vocal parameters and self-perception in individuals with adductor spasmodic dysphonia.
        J Voice. 2017; 31 (391.e7–391.e18)