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Hsa_circ_0081534 facilitates malignant phenotypes by sequestering miR-874-3p and upregulating FMNL3 in nasopharyngeal carcinoma

  • Author Footnotes
    1 Jintian He and Shunjin Chen contributed equally to this work.
    Jintian He
    Correspondence
    Corresponding author at: Department of Otorhinolaryngology, Dongguan People's Hospital, No.78, South Wanlu Road, xinguyong, Wanjiang District, Dongguan City, Guangdong 523059, China
    Footnotes
    1 Jintian He and Shunjin Chen contributed equally to this work.
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Author Footnotes
    1 Jintian He and Shunjin Chen contributed equally to this work.
    Shunjin Chen
    Footnotes
    1 Jintian He and Shunjin Chen contributed equally to this work.
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Xiaoying Wu
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Di Jiang
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Runqiu Li
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Zhiqiang Mao
    Affiliations
    Department of Otorhinolaryngology, Dongguan People's Hospital, Guangdong, 523059 China
    Search for articles by this author
  • Author Footnotes
    1 Jintian He and Shunjin Chen contributed equally to this work.
Published:April 12, 2022DOI:https://doi.org/10.1016/j.anl.2022.02.004

      Abstract

      Objective: Circular RNAs (circRNAs) are connected to nasopharyngeal carcinoma (NPC) development and progression. CircRNA hsa_circ_0081534 (circ_0081534) has been reported to be associated with NPC progression, but its underlying regulatory mechanisms are largely unknown. Thus, the study aims to investigate the mechanism by which circ_0081534 regulates NPC progression. Methods: Quantitative reverse transcription polymerase chain reaction was conducted to detect circ_0081534 expression. Loss-of-function assays were conducted to evaluate the role of circ_0081534, including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU) staining, colony formation, flow cytometry, transwell, western blotting, and xenograft assays. Targeting relationship was identified through dual-luciferase reporter assay and RNA immunoprecipitation. Results: Our data exhibited that circ_0081534 was upregulated in NPC samples and cells. Knockdown of circ_0081534 repressed NPC cell proliferation, migration, invasion, EMT, and triggered NPC cell apoptosis. Also, circ_0081534 silencing decreased NPC cell growth in xenograft models. Circ_0081534 functioned as a miR-874-3p sponge, and downregulation of miR-874-3p alleviated the suppressive effects of circ_0081534 silencing on NPC cell malignant phenotypes. MiR-874-3p targeted FMNL3, and circ_0081534 regulated FMNL3 expression through serving as a miR-874-3p sponge. Upregulation of FMNL3 relieved the inhibitory effects of circ_0081534 downregulation on NPC cell malignant phenotypes. Conclusion: circ_0081534 interference repressed NPC progression partly by modulating the miR-874-3p/FMNL3 axis.

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